HHS Publication No. (CDC) 93-8395 Biosafety in Microbiological and Biomedical Laboratories U.S. Department of Health and Human Services Public Health Service Centers for Disease Control and Prevention and National Institutes of Health 3rd Edition March 1993 U.S. GOVERNMENT PRINTING OFFICE WASHINGTON: 1993

Editors

CONTENTS

• Introduction

SECTION II

• Principles of Biosafety

TABLE 1. Summary of Recommended Biosafety Levels for Infectious Agents

TABLE 2. Summary of Recommended Biosafety Levels for Activities in Which
Experimentally or Naturally Infected Vertebrate Animals Are Used

SECTION III

• Laboratory Biosafety Level Criteria
  • BSL 1, BSL 2, BSL 3, BSL 4

  • TABLE 3. Comparison of Biological Safety Cabinets

SECTION IV

• Vertebrate Animal Biosafety Level Criteria
  • ABSL 1, ABSL 2, ABSL 3, ABSL 4

SECTION V

• Recommended Biosafety Levels For Infectious Agents and Infected Animals

SECTION VI

• Risk Assessment

SECTION VII

• Agent Summary Statements
  
• Parasitic Agents
  
• Fungal Agents
  
• Bacterial Agents
  
• Rickettsial Agents
  
• Viral Agents (other than arboviruses)
  
• Arboviruses
  
  • Arboviruses Assigned to Biosafety Level 2

    TABLE A. Arboviruses and Arenaviruses Assigned to Biosafety Level 2
    TABLE B. Vaccine Strains of BSL 3/4 Viruses Which May be Handled at BSL 2

  • Arboviruses and Arenaviruses Assigned to Biosafety Level 3
    TABLE C. Arboviruses and Certain Other Viruses Assigned to Biosafety Level 3
    TABLE D. Arboviruses and Certain Other Viruses Assigned to Biosafety Level 3

  • Arboviruses, Arenaviruses, or Filoviruses Assigned to Biosafety Level 4

• Biological Safety Cabinets

Figure 1.Class I Biological Safety Cabinet
Figure 2(a). Class II, Type A BSC
Figure 2(b). Class II, Type B1 BSC
Figure 2(c). Class II, Type B2 BSC
Figure 2(d). Table-top model of a Class II, Type B3 BSC
Figure 3.Class III BSC

APPENDIX B

• Immunoprophylaxis

APPENDIX C

• Surveillance of Personnel for Laboratory-Associated Rickettsial Infections

APPENDIX D

• Importation and Interstate Shipment of Human Pathogens and Related Materials

Figure 4. Packing and Labeling of Etiologic Agents

APPENDIX E

• Restricted Animal Pathogens

APPENDIX F

• Resources for Information

REFERENCES